Cell Lines and Cell Cultures
Eurofins Discoverx ChemiBrite A2A Adenosine Receptor Stable Cell Line, 2 vials. (HTS048L)
The ChemiBrite A2A Adenosine Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of ADORA2A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADORA2A alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of ADORA2A, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen NOP/ORL1 Opioid Receptor Membrane Preparation, 1 vial. (HTS040M)
The ChemiScreen NOP/ORL1 Opioid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of OPRL1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
![ABclonal Technology [KO Validated] CD133 Rabbit pAb](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000098397.jpg-250.jpg)
ABclonal Technology [KO Validated] CD133 Rabbit pAb
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
ATCC Dolosigranulum pigrum
Dolosigranulum pigrum strain NCFB 2975 is a bacterial type strain that was isolated from a human spinal cord during an autopsy in Colchester, England. This species is a potential emerging opportunistic pathogen with applications in infectious disease research.
ATCC Serratia marcescens
Depositors claim multidrug resistance to amikacin, aztreonam, carbenicillin, cefoperazone, cefotaxime, cefoxitin, chloramphenicol, ciprofloxacin, mezlocillin, piperacillin, tetracycline, and tobramycin (see reference). ATCC could confirm antibiotic resistance only to cefoxitin and tobramycin.
ATCC pRS306 phagemid; in E. coli
One of a series of pBluescript-based integrating vectors (ATCC 77138-77141) differing in the yeast selectable marker gene. This is a YI-type (integrating shuttle vector ) permitting visual detection of recombinants and production of ssDNA in E. coli. It contains promoters for invitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ?) peptide. The pRSS56, base vector was constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+). It contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and the f1 origin of pBS(+). A fragment (1.112 kb) containing the URA3 gene was inserted into the NdeI site of pRSS56. All ends were blunted.
Eurofins Discoverx PrecisION hKv4.3/hKChIP2 Recombinant Stable Cell Line, 2 vials. (CYL3069)
The PrecisION hKv4.3/hKChIP2 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Kv4.3/KChIP2 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Kv4.3/KChIP2 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
![ABclonal Technology [KO Validated] CD133 Rabbit pAb](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000098396.jpg-250.jpg)
ABclonal Technology [KO Validated] CD133 Rabbit pAb
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Eurofins Discoverx Ready-to-Assay MCH2 Melanin-Concentrating Hormone Receptor Frozen Cells, 2 Vials of Cells. (HTS026RTA)
The Ready-to-Assay MCH2 Melanin-concentrating Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. MCH) based activation of MCHR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MCHR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MCHR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay MCH1 Melanin-Concentrating Hormone Receptor Frozen Cells, 2 Vials of Cells. (HTS025RTA)
The Ready-to-Assay MCH1 Melanin-concentrating Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. MCH) based activation of MCHR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MCHR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MCHR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.